Christopher A Thomas, Jonathan M Craig, Shuichi Hoshika, Henry Brinkerhoff, Jesse R Huang, Sarah J Abell, Michaela C Franzi, Jessica D Carrasco, Hyo-Joong Kim, Drew C Smith, Jens H Gundlach, Steven A Benner, Andrew H Laszlo. "Assessing Readability of an 8-letter expanded deoxyribonucleic acid alphabet with nanopores." Jour. Amer. Chem. Soc. April 2023. https://doi.org/10.1021/jacs.3c00829
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There is significant interest in expanding the "alphabet" available in DNA with applications ranging from increased information storage density, to synthetic biology applications such as expanded codon libraries. Still, sequencing of DNA containing additional non-standard DNA bases is difficult and existing sequencing technologies are not easily adapted for sequencing an expanded DNA alphabet. Here we assess the feasibility of sequencing 8-letter DNA (consisting of canonical bases A, C, G, & T, and synthetic bases B, P, S, and Z) using nanopore DNA sequencing. We find that sequencing 8-letter DNA is a tractable problem.
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